This manual promotes the use of the groundbreaking human rights instrument, the Protocol on the Rights of Women in Africa. It is a guide that provides step-by-step guidance for using the Protocol at local, national, and regional levels. It explains how to bring women’s rights abuses that violate the Protocol before domestic courts and regional justice mechanisms like the African Court on Human and Peoples’ Rights. It also provides an analysis of certain violations of the Women’s Rights Protocol to assist practitioners in drafting complaints on these issues. The manual summarizes key cases on issues relevant to women’s rights decided by the African Commission and other relevant regional or international human rights mechanisms to give practitioners a sense of international and regional jurisprudence on women’s rights. It also highlights more general strategies that can be employed for the domestication and popularization of the Women’s Rights Protocol.